Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

I Stefanová; V Horejsí; H Kristofová; P Angelisová; V Zizkovský; I Hilgert

doi:10.1016/0022-1759(88)90060-9

Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

J Immunol Methods. 1988 Jun 28;111(1):67-73. doi: 10.1016/0022-1759(88)90060-9.

Authors

I Stefanová¹, V Horejsí, H Kristofová, P Angelisová, V Zizkovský, I Hilgert

Affiliation

¹ Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.

PMID: 2455753
DOI: 10.1016/0022-1759(88)90060-9

Abstract

Seven murine hybridomas were established producing IgG1 monoclonal antibodies (mAbs) against three different epitopes of human alpha-fetoprotein (alpha FP). The interaction of two of these mAbs (AFP-01 and AFP-02) with the antigen was strongly inhibited by calcium-chelating agents. The mAb AFP-11 could be used for estimation of alpha FP by RIA in the concentration range of 5-100 ng/ml. Several combinations of the mAbs could be used in a two-site sandwich-ELISA for similar purposes. Immunoaffinity chromatography on a column of immobilized mAb AFP-01 permitted the purification of alpha FP.

MeSH terms

Animals
Antibodies, Monoclonal* / biosynthesis
Antibodies, Monoclonal* / immunology
Antigen-Antibody Reactions / drug effects
Binding, Competitive
Chelating Agents / pharmacology
Collodion
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Epitopes / immunology
Female
Humans
Hybridomas / metabolism
Indicators and Reagents
Mice
Mice, Inbred BALB C
Radioimmunoassay
Structure-Activity Relationship
alpha-Fetoproteins / analysis
alpha-Fetoproteins / immunology*

Substances

Antibodies, Monoclonal
Chelating Agents
Epitopes
Indicators and Reagents
alpha-Fetoproteins
Collodion