Abstract
Id1 and NF-κB are highly expressed in oral squamous cell carcinoma (OSCC). Whether they have a synergistic role in the carcinogenesis of OSCC is unclear. The current study was designed to demonstrate the synergy of both Id1 and NF-κB in the underlying disease mechanisms of OSCC using in vitro and in vivo animal models. Id1 and NF-κB strengthened the expression of both CD133 and BMI-1 in OSCC cell cultures. CD133(+) and BMI-1(+) keratinocytes from OSCC tissues and cell cultures initiated the growth of xenograft tumors in SCID/Beige mice. Id1 and NF-κB regulate the expression of CD133 and BMI-1 in an additive or synergistic manner in OSCC, which is associated with the generation of naïve and self-renewable keratinocytes and initiate the growth of xenograft tumors in vivo.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
AC133 Antigen
-
Animals
-
Antigens, CD / metabolism*
-
Carcinoma, Squamous Cell / genetics*
-
Carcinoma, Squamous Cell / pathology
-
Cell Line, Tumor
-
Cell Proliferation
-
Glycoproteins / metabolism*
-
Head and Neck Neoplasms / genetics*
-
Head and Neck Neoplasms / pathology
-
Heterografts / pathology
-
Humans
-
Inhibitor of Differentiation Protein 1 / genetics
-
Inhibitor of Differentiation Protein 1 / metabolism*
-
Keratinocytes / metabolism*
-
Mice
-
Mice, SCID
-
Mouth Neoplasms / genetics*
-
Mouth Neoplasms / pathology
-
Neoplasm Transplantation / pathology
-
Peptides / metabolism*
-
Polycomb Repressive Complex 1 / metabolism*
-
Transcription Factor RelA / genetics
-
Transcription Factor RelA / metabolism*
Substances
-
AC133 Antigen
-
Antigens, CD
-
Glycoproteins
-
Inhibitor of Differentiation Protein 1
-
PROM1 protein, human
-
Peptides
-
Prom1 protein, mouse
-
Transcription Factor RelA
-
Polycomb Repressive Complex 1