Enhanced intracellular siRNA delivery using bioreducible lipid-like nanoparticles

Adv Healthc Mater. 2014 Sep;3(9):1398-403. doi: 10.1002/adhm.201400039. Epub 2014 Feb 20.

Abstract

A new library of lipid-like nanoparticles (lipidoids) comprising disulfide bond is developed for siRNA delivery. Bioreducible lipidoids deliver siRNA with greater efficiency than nonbioreducible lipidoids with similar chemical structures. A siRNA release investigation, as well as an intracellular siRNA trafficking study, reveals that the degradation of bioreducible lipidoid in a strongly reductive intracellular environment boosts siRNA release and enhances siRNA gene knockdown efficiency.

Keywords: bioreducible lipidoid; cancer therapy; combinatorial; nanoparticles; siRNA delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Gene Knockdown Techniques / methods*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Humans
  • Intracellular Space / chemistry
  • Intracellular Space / metabolism*
  • Lipids / chemistry
  • Lipids / pharmacokinetics*
  • Nanoparticles / chemistry
  • Nanoparticles / metabolism*
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism*
  • Transfection / methods

Substances

  • Lipids
  • RNA, Small Interfering
  • Green Fluorescent Proteins