Abstract
Some bacterial infections involve potentially complex mixtures of species that can now be distinguished using next-generation DNA sequencing. We present a case of mastoiditis where Gram stain, culture, and molecular diagnosis were nondiagnostic or discrepant. Next-generation sequencing implicated coinfection of Fusobacterium nucleatum and Actinomyces israelii, resolving these diagnostic discrepancies.
Publication types
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Case Reports
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Research Support, N.I.H., Extramural
MeSH terms
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Actinomyces / isolation & purification*
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Actinomycosis / diagnosis
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Actinomycosis / microbiology
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Coinfection / diagnosis*
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Coinfection / microbiology*
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Fusobacterium Infections / diagnosis
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Fusobacterium Infections / microbiology
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Fusobacterium nucleatum / genetics
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Fusobacterium nucleatum / isolation & purification*
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High-Throughput Nucleotide Sequencing / methods
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Humans
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Male
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Mastoiditis / diagnosis*
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Mastoiditis / microbiology*
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Middle Aged