Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory

M J Buitrago; L Bernal-Martinez; M V Castelli; J L Rodriguez-Tudela; M Cuenca-Estrella

doi:10.1128/JCM.00328-14

Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory

J Clin Microbiol. 2014 May;52(5):1737-40. doi: 10.1128/JCM.00328-14. Epub 2014 Feb 26.

Authors

M J Buitrago¹, L Bernal-Martinez, M V Castelli, J L Rodriguez-Tudela, M Cuenca-Estrella

Affiliation

¹ Mycology Department, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Abstract

A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biopsy / methods
DNA, Fungal / genetics
Fungi / genetics*
Humans
Mycoses / diagnosis*
Mycoses / microbiology*
Polymerase Chain Reaction / methods
Retrospective Studies

Substances

DNA, Fungal