Abstract
To examine the nature of the interaction of the TCR with the MHC class I Ag, we have studied the stimulation requirements of an H-2Dd-reactive T cell hybridoma, using a homogeneous, purified preparation of a molecularly engineered soluble counterpart of the class I Ag, H-2Dd/Q10b. We demonstrate that this monovalent, soluble MHC Ag is incapable of stimulating the release of IL-2 from this T cell hybridoma. However, the same preparation of the purified protein can elicit a dose-dependent response when made multivalent either by covalent coupling to soluble, high m.w. dextran or to agarose beads, or by adsorption to polystyrene tissue culture plates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / physiology
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Antigens, Surface / genetics
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Antigens, Surface / immunology*
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Binding Sites, Antibody
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Binding, Competitive
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Cross-Linking Reagents
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Dextrans
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H-2 Antigens / genetics
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H-2 Antigens / immunology*
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Histocompatibility Antigens / genetics
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Histocompatibility Antigens / immunology*
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Histocompatibility Antigens Class I*
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Hybridomas / immunology
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Hybridomas / metabolism
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Interleukin-2 / biosynthesis
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Lymphocyte Activation*
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Mice
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology*
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism*
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Transfection
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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Cross-Linking Reagents
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Dextrans
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H-2 Antigens
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Histocompatibility Antigens
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Histocompatibility Antigens Class I
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Interleukin-2
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Q surface antigens
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Receptors, Antigen, T-Cell