Abstract
Antiviral effects were characterized for two oligodeoxyribonucleoside methylphosphonates synthesized in an antisense (3'-TCTTAACC-5') or a sense (5'-AGAATTGG-3') orientation, based on the RNA sequence of the first splice acceptor site of the tat-3 gene of human immunodeficiency virus (HIV) (5'...AGAAUUGG...3'). The development of syncytial cells and supernatant reverse transcriptase was inhibited by a single exposure to the antisense HIV, and HIV RNA synthesis was inhibited by both antisense and sense methylphosphonates but not by a control herpes simplex virus antisense sequence.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Antiviral Agents / pharmacology*
-
Cytopathogenic Effect, Viral
-
Deoxyribonucleotides / pharmacology*
-
Gene Products, tat
-
Genes, Viral / drug effects*
-
HIV / drug effects*
-
HIV / enzymology
-
HIV / genetics
-
HIV / physiology
-
Humans
-
Oligodeoxyribonucleotides*
-
RNA, Viral / biosynthesis
-
RNA, Viral / drug effects*
-
RNA-Directed DNA Polymerase / biosynthesis
-
Transcription Factors / genetics*
-
Virus Replication / drug effects
-
tat Gene Products, Human Immunodeficiency Virus
Substances
-
Antiviral Agents
-
Deoxyribonucleotides
-
Gene Products, tat
-
Oligodeoxyribonucleotides
-
RNA, Viral
-
Transcription Factors
-
dideoxyribonucleoside methylphosphonates
-
tat Gene Products, Human Immunodeficiency Virus
-
RNA-Directed DNA Polymerase