In situ staining of bromodeoxyuridine positive cells in normal and neoplastic colony-forming units grown on plasma clots

J Immunol Methods. 1988 Oct 26;113(2):215-9. doi: 10.1016/0022-1759(88)90334-1.

Abstract

Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In this paper a method for the detection of the labeling index of normal and neoplastic colony-forming units (CFU) growing in plasma clot semisolid medium is described and preliminary results on the cell cycle of 7th and 14th CFU granulocyte-macrophage are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Coagulation
  • Bromodeoxyuridine*
  • Cell Aggregation
  • Cell Division
  • Cell Line
  • Clone Cells
  • Colony-Forming Units Assay*
  • Hematopoietic Stem Cells / analysis*
  • Hematopoietic Stem Cells / pathology
  • Humans
  • Neoplastic Stem Cells / analysis*
  • Neoplastic Stem Cells / pathology
  • Plasma
  • Staining and Labeling* / methods

Substances

  • Bromodeoxyuridine