Post-maturation zona perforation improves porcine parthenogenetic trophoblast culture

I M Saadeldin; S J Kim; Y B Choi; B C Lee

doi:10.1016/j.placenta.2014.02.003

Post-maturation zona perforation improves porcine parthenogenetic trophoblast culture

Placenta. 2014 Apr;35(4):286-8. doi: 10.1016/j.placenta.2014.02.003. Epub 2014 Feb 18.

Authors

I M Saadeldin¹, S J Kim², Y B Choi², B C Lee³

Affiliations

¹ Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
² Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
³ Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang, Kangwon do, Republic of Korea. Electronic address: [email protected].

PMID: 24593992
DOI: 10.1016/j.placenta.2014.02.003

Abstract

This study was designed to optimize a method to improve porcine parthenogenetic embryo hatching and trophoblast culture. Mature oocytes (D0PPA) and day 6 blastocysts (D6PPA) were perforated with a 20 μm diameter needle for assisted hatching. The two groups showed a significant difference in hatching rate and blastocyst cell doubling when compared to a non-perforated control group. D0PPA blastocysts were able to form tertiary trophoblast colonies but D6PPA and control groups were not able to grow beyond primary colonies. Quantitative real-time PCR analysis showed significant differences in BAX, BAX/BCL2L1 and HSP70-2 mRNA expression between the experimental groups.

Keywords: Parthenogenesis; Pig; Trophoblast outgrowth; Zona perforation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques
Embryo Culture Techniques*
Female
Oocytes
Parthenogenesis
Swine
Trophoblasts*