Abstract
Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12), consistent with 6.46 coverage of the tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the tiger.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Line
-
Chromosomes, Artificial, Bacterial / genetics*
-
Endangered Species
-
Genome / genetics*
-
Genomic Library*
-
Genomics / methods
-
Microsatellite Repeats / genetics
-
Molecular Sequence Data
-
Sequence Analysis, DNA
-
Tigers / genetics*
Associated data
-
GENBANK/KF463188
-
GENBANK/KF463189
-
GENBANK/KF463190
-
GENBANK/KF463191
-
GENBANK/KF463192
-
GENBANK/KF463193
-
GENBANK/KF463194
-
GENBANK/KF463195
-
GENBANK/KF463196
-
GENBANK/KF463197
-
GENBANK/KF463198
-
GENBANK/KF463199
-
GENBANK/KF463200
-
GENBANK/KF463201
-
GENBANK/KF463202
-
GENBANK/KF463203
-
GENBANK/KF463204
-
GENBANK/KF463205
-
GENBANK/KF463206
-
GENBANK/KF463207
-
GENBANK/KF463208
-
GENBANK/KF463209
-
GENBANK/KF463210
-
GENBANK/KF463211
-
GENBANK/KF463212
-
GENBANK/KF463213
-
GENBANK/KF463214
-
GENBANK/KF463215
-
GENBANK/KF463216
-
GENBANK/KF463217
-
GENBANK/KF463218
-
GENBANK/KF463219
-
GENBANK/KF463220
-
GENBANK/KF463221
-
GENBANK/KF463222
-
GENBANK/KF463223
-
GENBANK/KF463224
-
GENBANK/KF463225
-
GENBANK/KF463226
-
GENBANK/KF463227
-
GENBANK/KF463228
-
GENBANK/KF463229
-
GENBANK/KF463230
-
GENBANK/KF463231
-
GENBANK/KF463232
-
GENBANK/KF463233
-
GENBANK/KF463234
-
GENBANK/KF463235
-
GENBANK/KF463236
-
GENBANK/KF463237
-
GENBANK/KF463238
-
GENBANK/KF463239
-
GENBANK/KF463240
-
GENBANK/KF463241
-
GENBANK/KF463242
-
GENBANK/KF463243
-
GENBANK/KF463244
-
GENBANK/KF463245
-
GENBANK/KF463246
-
GENBANK/KF463247
-
GENBANK/KF463248
-
GENBANK/KF463249
-
GENBANK/KF463250
-
GENBANK/KF463251
-
GENBANK/KF463252
-
GENBANK/KF463253
-
GENBANK/KF463254
-
GENBANK/KF463255
-
GENBANK/KF463256
-
GENBANK/KF463257
-
GENBANK/KF463258
-
GENBANK/KF463259