CMV infection, TLR-2 and -4 expression, and cytokine profiles in early-onset preeclampsia with HELLP syndrome

Am J Reprod Immunol. 2014 Apr;71(4):379-86. doi: 10.1111/aji.12199.

Abstract

Problem: Cytomegalovirus (CMV) infection was previously reported in pregnancy complications. However, its seroprevalence and associated Toll-like receptor (TLR) expression in early-onset preeclampsia (EOPE) with hemolysis, elevated liver enzyme and low platelets syndrome (HELLPs) are unexplored.

Method of study: A case–control study was performed to examine maternal CMV antibodies, neutrophil Toll-like receptor (TLR)-2 and -4 expression as well as the cytokine profile in EOPE with HELLPs (EOPE-HELLPs) (n = 10), late-onset preeclampsia (LOPE) (n = 20), normal pregnancy (n = 60), and non-pregnancy (n = 20) controls.

Results: EOPE-HELLPs had significantly increased CMV IgG sero-positivity, upregulated TLR-2/-4 mRNA expression, increased serum IL-6 and TNF-α, and reduced IL-10 compared with matched normal and non-pregnancy controls. No significant difference was observed between LOPE and normal pregnancy controls.

Conclusion: We observed a significant association between CMV IgG sero-positivity and innate immune response in EOPE-HELLPs. Our data suggest that CMV infection may be a risk factor for this disorder.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Viral / blood
  • Case-Control Studies
  • Cytokines / biosynthesis
  • Cytomegalovirus / immunology
  • Cytomegalovirus / pathogenicity
  • Cytomegalovirus Infections / immunology*
  • Cytomegalovirus Infections / pathology
  • Female
  • HELLP Syndrome / immunology
  • HELLP Syndrome / pathology*
  • Humans
  • Immunity, Innate
  • Immunoglobulin G / blood
  • Interleukin-10 / blood
  • Interleukin-6 / blood
  • Neutrophils / immunology
  • Pre-Eclampsia / immunology
  • Pre-Eclampsia / pathology*
  • Pregnancy
  • Pregnancy Complications, Hematologic / immunology
  • Pregnancy Complications, Infectious / immunology
  • Pregnancy Complications, Infectious / virology
  • RNA, Messenger / biosynthesis
  • Toll-Like Receptor 2 / biosynthesis*
  • Toll-Like Receptor 2 / genetics
  • Toll-Like Receptor 4 / biosynthesis*
  • Toll-Like Receptor 4 / genetics
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Antibodies, Viral
  • Cytokines
  • IL10 protein, human
  • IL6 protein, human
  • Immunoglobulin G
  • Interleukin-6
  • RNA, Messenger
  • TLR2 protein, human
  • TLR4 protein, human
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Tumor Necrosis Factor-alpha
  • Interleukin-10