The flavonols quercetin and 3',4'-dihydroxyflavonol reduce platelet function and delay thrombus formation in a model of type 1 diabetes

Diab Vasc Dis Res. 2014 May;11(3):174-81. doi: 10.1177/1479164114524234. Epub 2014 Mar 12.

Abstract

Diabetes is associated with increased cardiovascular risk. We have recently shown that the naturally occurring flavonol quercetin (Que) or the synthetic flavonol 3',4'-dihydroxyflavonol (DiOHF) inhibits platelet function and delays thrombus formation in healthy mice. Therefore, the aim of this study was to investigate the effect of Que or DiOHF treatment on platelet function and ferric chloride-induced carotid artery thrombosis in a mouse model of type 1 diabetes. Diabetic mice treated with Que or DiOHF maintained blood flow at a significantly higher level than untreated diabetic mice at the end of the recording period. In addition, treatment with Que or DiOHF significantly reduced diabetes-induced platelet hyper-aggregability in response to platelet agonist stimulation. Furthermore, treatment with Que or DiOHF significantly inhibited dense, but not alpha, granule exocytosis in diabetic and control mice. Our demonstration that flavonols delay thrombus formation in diabetes suggests a potential clinical role for these compounds in anti-platelet therapy.

Keywords: 3′,4′-dihydroxyflavonol; Diabetes; flavonol; platelet; quercetin; thrombosis.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Blood Platelets / drug effects*
  • Carotid Arteries / drug effects*
  • Carotid Artery Injuries / chemically induced
  • Chlorides / toxicity
  • Diabetes Mellitus, Experimental*
  • Diabetes Mellitus, Type 1*
  • Disease Models, Animal
  • Exocytosis / drug effects
  • Ferric Compounds / toxicity
  • Flavonols / pharmacology*
  • Laser-Doppler Flowmetry
  • Mice
  • Mice, Inbred C57BL
  • Platelet Activation / drug effects
  • Platelet Aggregation / drug effects*
  • Quercetin / pharmacology*
  • Thrombosis / prevention & control*

Substances

  • Antioxidants
  • Chlorides
  • Ferric Compounds
  • Flavonols
  • Quercetin
  • 3',4'-dihydroxyflavonol
  • ferric chloride