Vectorology and factor delivery in induced pluripotent stem cell reprogramming

Stem Cells Dev. 2014 Jun 15;23(12):1301-15. doi: 10.1089/scd.2013.0621. Epub 2014 Apr 16.

Abstract

Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncontrolled silencing, residual expression and re-activation of transgenes, and immunogenicity. To overcome these issues, various technologies were explored, including adenoviral vectors, protein transduction, RNA transfection, minicircle DNA, excisable PiggyBac (PB) transposon, Cre-lox excision system, negative-sense RNA replicon, positive-sense RNA replicon, Epstein-Barr virus-based episomal plasmids, and repeated transfections of plasmids. This review provides summaries of the main vectorologies and factor delivery systems used in current reprogramming protocols.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Culture Techniques / methods
  • Cell Differentiation
  • Cellular Reprogramming*
  • DNA Transposable Elements / genetics
  • Genetic Vectors*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Mutagenesis, Insertional
  • Retroviridae / genetics*
  • Transfection

Substances

  • DNA Transposable Elements