Identification of isoafricanol and its terpene cyclase in Streptomyces violaceusniger using CLSA-NMR

Chem Commun (Camb). 2014 Apr 25;50(32):4228-30. doi: 10.1039/c4cc00177j.

Abstract

The recently developed CLSA-NMR technique that is based on feeding experiments with (13)C-labelled precursors was applied in the identification of isoafricanol as the main volatile terpene emitted by Streptomyces violaceusniger. The isoafricanol synthase of this organism is presented, together with a recent phylogenetic analysis of bacterial terpene cyclases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / isolation & purification
  • Alkyl and Aryl Transferases / metabolism*
  • Gas Chromatography-Mass Spectrometry / methods*
  • Magnetic Resonance Spectroscopy / methods*
  • Solid Phase Microextraction / methods*
  • Streptomyces / classification
  • Streptomyces / metabolism*
  • Terpenes / analysis*
  • Terpenes / metabolism*

Substances

  • Terpenes
  • Alkyl and Aryl Transferases
  • terpene carbocyclase