Purification of O2-sensitive metalloproteins

Carlos Echavarri-Erasun; Simon Arragain; Luis M Rubio

doi:10.1007/978-1-62703-794-5_2

Purification of O2-sensitive metalloproteins

Methods Mol Biol. 2014:1122:5-18. doi: 10.1007/978-1-62703-794-5_2.

Authors

Carlos Echavarri-Erasun¹, Simon Arragain, Luis M Rubio

Affiliation

¹ Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de, Alarcón, Madrid, 28223, Spain.

PMID: 24639250
DOI: 10.1007/978-1-62703-794-5_2

Abstract

The most dependable factor to perform successful biochemical experiments in an O2-free environment is the experience required to set up an efficient laboratory, to properly manipulate samples, to anticipate potential O2-related problems, and to maintain the complex laboratory setup operative. There is a long list of O2-related issues that may ruin your experiments. We provide here a guide to minimize these risks.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anaerobiosis / drug effects
Biochemistry / methods*
Buffers
Chromatography, High Pressure Liquid
Dithionite / pharmacology
Free Radical Scavengers / pharmacology
Metalloproteins / isolation & purification*
Metalloproteins / metabolism*
Oxygen / metabolism*
Reducing Agents / pharmacology

Substances

Buffers
Free Radical Scavengers
Metalloproteins
Reducing Agents
Dithionite
Oxygen