Karyotype divergence and spreading of 5S rDNA sequences between genomes of two species: darter and emerald gobies ( Ctenogobius , Gobiidae)

Cytogenet Genome Res. 2014;142(3):197-203. doi: 10.1159/000360492. Epub 2014 Mar 13.

Abstract

Karyotype analyses of the cryptobenthic marine species Ctenogobius boleosoma and C. smaragdus were performed by means of classical and molecular cytogenetics, including physical mapping of the multigene 18S and 5S rDNA families. C. boleosoma has 2n = 44 chromosomes (2 submetacentrics + 42 acrocentrics; FN = 46) with a single chromosome pair each carrying 18S and 5S ribosomal sites; whereas C. smaragdus has 2n = 48 chromosomes (2 submetacentrics + 46 acrocentrics; FN = 50), also with a single pair bearing 18S rDNA, but an extensive increase in the number of GC-rich 5S rDNA sites in 21 chromosome pairs. The highly divergent karyotypes among Ctenogobius species contrast with observations in several other marine fish groups, demonstrating an accelerated rate of chromosomal evolution mediated by both chromosomal rearrangements and the extensive dispersion of 5S rDNA sequences in the genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Aberrations / veterinary*
  • Chromosome Banding / veterinary
  • Chromosome Mapping / veterinary
  • DNA, Ribosomal / genetics*
  • Evolution, Molecular*
  • Fishes / genetics*
  • Genetic Variation
  • Karyotype
  • RNA, Ribosomal, 18S / genetics
  • RNA, Ribosomal, 5S / genetics
  • Sequence Analysis, RNA

Substances

  • DNA, Ribosomal
  • RNA, Ribosomal, 18S
  • RNA, Ribosomal, 5S