Abstract
Dengue fever (DF) and dengue hemorrhagic fever (DHF) are caused by mosquito-borne dengue virus (DENV) infection leading to death in tropical and subtropical countries. In Thailand, all 4 serotypes of DENV are circulating. The most severe cases of DF and DHF are primarily introduced by secondary infections. Epidemiological studies have demonstrated that approximately 20% of the primary infection cases were caused by DENV-1 and -3, while the cases of DENV-2 or -4 accounted for less than 3%. For this reason, DENV-2 and -4 from primary infections have not been well studied. In this study, the sequence diversity of the envelope gene of 8 DENV-2 clinical isolates from primary/secondary infections was analyzed. DENV-2 from primary infections were highly heterogeneous in individual patients, whereas those from secondary infections were homogeneous. Phylogenetic analysis demonstrated that the heterogeneous population of DENV-2 from primary infections was composed of closely related quasispecies. Homogenous DENV-2 could be derived from selection of a particular viral population in secondary infections. The degree of sequence diversity of DENV-2 varied, and thus quasispecies may be involved in the progression of DENV infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cluster Analysis
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Dengue / virology*
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Dengue Virus / classification*
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Dengue Virus / genetics
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Dengue Virus / isolation & purification*
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Genetic Variation*
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Genotype
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Humans
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Molecular Sequence Data
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Phylogeny
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RNA, Viral / genetics
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Sequence Analysis, DNA
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Sequence Homology
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Thailand
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Viral Envelope Proteins / genetics
Substances
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RNA, Viral
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Viral Envelope Proteins
Associated data
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GENBANK/AB587891
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GENBANK/AB587892
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GENBANK/AB587893
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GENBANK/AB587894
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GENBANK/AB587895
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GENBANK/AB587896
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