Iloprost-induced translocation of a 23-kDa protein that is recognized by a Gs alpha antiserum

Proc Natl Acad Sci U S A. 1989 Feb;86(3):868-70. doi: 10.1073/pnas.86.3.868.

Abstract

We have observed that iloprost, a prostacyclin analog, causes the translocation of a 23-kDa protein from the membrane to the cytosol of human platelets. This 23-kDa protein is recognized by a Gs alpha (alpha subunit of stimulatory guanine nucleotide binding regulatory protein) antiserum but is not ADP-ribosylated by cholera toxin. The translocation of the Gs alpha-related protein might be an important part of the complex chain of events resulting in agonist-induced desensitization.

MeSH terms

  • Blood Platelets / metabolism*
  • Cardiovascular Agents / pharmacology
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cytosol / metabolism
  • Epoprostenol / pharmacology*
  • GTP-Binding Proteins / blood*
  • GTP-Binding Proteins / immunology
  • GTP-Binding Proteins / isolation & purification
  • Humans
  • Iloprost
  • Immune Sera
  • Macromolecular Substances
  • Molecular Weight

Substances

  • Cardiovascular Agents
  • Immune Sera
  • Macromolecular Substances
  • Epoprostenol
  • GTP-Binding Proteins
  • Iloprost