Abstract
Recent studies have demonstrated that small double-stranded RNAs (dsRNAs) complementary to the promoter region of target genes enhance the expression of those genes following transfection into cells. Here we show that expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with dsRNA complementary to the promoter of the RECK gene, leading to suppression of the expression of MMPs and it inhibited tumor cell invasion. These results support the suggestion that dsRNA complementary to the promoter region of tumor suppressor genes would have potential as a novel antitumor agent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Adenocarcinoma / therapy
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Adenocarcinoma of Lung
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Base Sequence
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Breast Neoplasms / therapy
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Carcinoma, Non-Small-Cell Lung / genetics
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Carcinoma, Non-Small-Cell Lung / metabolism
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Non-Small-Cell Lung / therapy
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Cell Line, Tumor
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Female
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GPI-Linked Proteins / biosynthesis
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GPI-Linked Proteins / genetics*
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GPI-Linked Proteins / metabolism
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Gene Expression Regulation, Neoplastic
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Genetic Therapy / methods*
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Humans
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Lung Neoplasms / therapy
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Molecular Sequence Data
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Promoter Regions, Genetic
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RNA Interference
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RNA, Double-Stranded / genetics
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Transfection / methods
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Up-Regulation
Substances
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GPI-Linked Proteins
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RECK protein, human
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RNA, Double-Stranded