Single nucleotide polymorphisms of microRNA processing machinery genes and outcome of hepatocellular carcinoma

PLoS One. 2014 Mar 27;9(3):e92791. doi: 10.1371/journal.pone.0092791. eCollection 2014.

Abstract

MicroRNA (miRNA)-related single nucleotide polymorphisms (miR-SNPs) can affect cancer development, treatment efficacy and patients prognosis. We examined 6 miR-SNPs in miRNA processing machinery genes including exportin 5 (XPO5) (rs11077), Ran-GTPase (RAN) (rs14035), Dicer (rs3742330), Trinucleotide Repeat Containing 6B (TNRC6B) (rs9623117), GEMIN3 (rs197412), GEMIN4 (rs2740348) in 108 surgically resected HCC patients and evaluated the impact of these miR-SNPs on HCC outcome. Among the 6 SNPs, only the A/A genotype of rs11077 located in XPO5 3'UTR was identified to associated independently with worse survival in HCC patients by multivariate analysis with relative risk, 0.395; 95% CI, 0.167-0.933; p = 0.034. This is the first study reporting that polymorphisms related to miRSNPs have prognostic value in hepatocellular carcinoma and identify the A/A genotype of rs11077 SNP site located in XPO5 3'UTR can help to predict worse prognosis in patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / mortality*
  • Carcinoma, Hepatocellular / pathology
  • Female
  • Follow-Up Studies
  • Genotype
  • Humans
  • Kaplan-Meier Estimate
  • Karyopherins / genetics
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / mortality*
  • Liver Neoplasms / pathology
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Staging
  • Patient Outcome Assessment
  • Polymorphism, Single Nucleotide*
  • Prognosis

Substances

  • Karyopherins
  • MicroRNAs
  • XPO5 protein, human

Grants and funding

SL is supported by Bethune International Peace Hospital (BIPH) research grant. JL is supported by SPORE career development award from Dana-Farber Cancer Institute (DFCI). JJZ is supported by Multiple Myeloma Research Foundation (MMRF) award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.