FASN expression, angiogenesis and lymphangiogenesis in central and peripheral giant cell lesions

J Appl Oral Sci. 2014 Apr;22(2):131-7. doi: 10.1590/1678-775720130509.

Abstract

Central giant cell lesion (CGCL) and peripheral giant cell lesion (PGCL) are non-neoplastic proliferative processes of the jaws. PGCL is a reactive process induced by irritant local factors and CGCL is an intra-osseous lesion of unknown etiology. Both lesions exhibit similar histologic features showing abundant mononuclear cells, admixed with a large number of multinucleated giant cells and a rich vascularized stroma with extravasated erythrocytes, hemosiderin deposition, and blood-filled pools. Recent studies have linked fatty acid synthase (FASN) with angiogenesis.

Objective: To evaluate angiogenesis and lymphangiogenesis and their relationship with FASN expression in CGCL and PGCL.

Material and methods: Thirteen CGCL and 14 PGCL of the jaws were selected for immunoexpression of FASN; CD34 and CD105 (to assess blood microvessel density [MVD] and microvessel area [MVA]); and D2-40 (to assess lymphatic MVD and MVA).

Results: Within PGCL and CGCL, MVD-CD34 was signifcantly higher than MVD-CD10S, followed by MVD-D2-40. Moreover, a signifcantly higher number of FASN-positive multinucleated giant cells than mononuclear cells were observed. Between PGCL and CGCL, only MVD-CD34 and all MVA were signifcantly higher in PGCL. Positive correlation between MVA-CD10S with FASNpositive mononuclear cells in both lesions was observed.

Conclusions: Our results show both lesions exhibiting similar levels of FASN expression and neoangiogenesis, suggesting constitutive processes that regulate tissue maintenance.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antigens, CD / analysis
  • Antigens, CD34 / analysis
  • Biopsy
  • Endoglin
  • Fatty Acid Synthase, Type I / analysis*
  • Female
  • Giant Cells / pathology*
  • Humans
  • Immunohistochemistry
  • Jaw Diseases / pathology*
  • Lymphangiogenesis / physiology*
  • Male
  • Microvessels / pathology
  • Neovascularization, Pathologic / pathology*
  • Receptors, Cell Surface / analysis
  • Retrospective Studies
  • Statistics, Nonparametric
  • Young Adult

Substances

  • Antigens, CD
  • Antigens, CD34
  • ENG protein, human
  • Endoglin
  • Receptors, Cell Surface
  • FASN protein, human
  • Fatty Acid Synthase, Type I