IL2/IL-4, OX40L and FDC-like cell line support the in vitro tumor cell growth of adult T-cell leukemia/lymphoma

Dai Chihara; Yoshitoyo Kagami; Harumi Kato; Noriaki Yoshida; Tohru Kiyono; Yasutaka Okada; Tomohiro Kinoshita; Masao Seto

doi:10.1016/j.leukres.2014.03.003

IL2/IL-4, OX40L and FDC-like cell line support the in vitro tumor cell growth of adult T-cell leukemia/lymphoma

Leuk Res. 2014 May;38(5):608-12. doi: 10.1016/j.leukres.2014.03.003. Epub 2014 Mar 12.

Authors

Dai Chihara¹, Yoshitoyo Kagami², Harumi Kato³, Noriaki Yoshida⁴, Tohru Kiyono⁵, Yasutaka Okada⁶, Tomohiro Kinoshita³, Masao Seto⁴

Affiliations

¹ Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
² Department of Hematology, Toyota Kosei Hospital, Toyota, Japan. Electronic address: [email protected].
³ Department of Haematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.
⁴ Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
⁵ Division of Virology, National Cancer Center Research Institute, Tokyo, Japan.
⁶ Department of Clinical Laboratories, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.

PMID: 24679586
DOI: 10.1016/j.leukres.2014.03.003

Abstract

Adult T-cell leukemia/lymphoma (ATLL) is a peripheral T-cell neoplasm with an extremely poor prognosis. Maintaining ATLL cells in vitro is difficult and little is known about how they maintain themselves or grow in patients. Elucidating the interaction between ATLL cells and surrounding host factors might therefore provide important insights into pathophysiology. We cultured primary ATLL cells in various culture conditions using IL-2, IL-4 and feeder cells, and established two cell lines dependent on IL-2, IL-4 and a follicular dendritic cell-derived cell line, HK, in which OX40-ligand was induced. Our study indicates the importance of microenvironment in the homeostasis of ATLL.

Keywords: Adult T-cell leukemia/lymphoma; Cell line; Microenvironment.

MeSH terms

Adult
Aged
Cell Line, Tumor
Cell Proliferation*
Dendritic Cells, Follicular / physiology*
Female
Genotype
Humans
Interleukin-2 / physiology*
Interleukin-4 / physiology*
Leukemia-Lymphoma, Adult T-Cell / pathology*
Male
OX40 Ligand / physiology*
Tumor Microenvironment

Substances

IL4 protein, human
Interleukin-2
OX40 Ligand
TNFSF4 protein, human
Interleukin-4