Two photon absorption energy transfer in the light-harvesting complex of photosystem II (LHC-II) modified with organic boron dye

Li Chen; Cheng Liu; Rui Hu; Jiao Feng; Shuangqing Wang; Shayu Li; Chunhong Yang; Guoqiang Yang

doi:10.1016/j.saa.2014.02.166

Two photon absorption energy transfer in the light-harvesting complex of photosystem II (LHC-II) modified with organic boron dye

Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15:128:295-9. doi: 10.1016/j.saa.2014.02.166. Epub 2014 Mar 12.

Authors

Li Chen¹, Cheng Liu², Rui Hu¹, Jiao Feng¹, Shuangqing Wang³, Shayu Li¹, Chunhong Yang⁴, Guoqiang Yang⁵

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
² Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
³ Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: [email protected].
⁴ Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: [email protected].
⁵ Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: [email protected].

PMID: 24681315
DOI: 10.1016/j.saa.2014.02.166

Abstract

The plant light-harvesting complexes of photosystem II (LHC-II) play important roles in collecting solar energy and transferring the energy to the reaction centers of photosystems I and II. A two photon absorption compound, 4-(bromomethyl)-N-(4-(dimesitylboryl)phenyl)-N-phenylaniline (DMDP-CH2Br), was synthesized and covalently linked to the LHC-II in formation of a LHC-II-dye complex, which still maintained the biological activity of LHC-II system. Under irradiation with femtosecond laser pulses at 754 nm, the LHC-II-dye complex can absorb two photons of the laser light effectively compared with the wild type LHC-II. The absorbed excitation energy is then transferred to chlorophyll a with an obvious fluorescence enhancement. The results may be interesting and give potentials for developing hybrid photosystems.

Keywords: Energy transfer; LHC-II; Light-harvesting complex of photosystem II; Organic boron dye; Two photon absorption.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Absorptiometry, Photon / methods
Aniline Compounds / chemistry*
Boron Compounds / chemistry*
Chlorophyll / chemistry*
Fluorescence*
Photosystem II Protein Complex / chemistry*

Substances

Aniline Compounds
Boron Compounds
Photosystem II Protein Complex
Chlorophyll