Evaluation of the microvessel density and the expression of metalloproteases 2 and 9 and ttf1 in the different subtypes of lung adenocarcinoma in Tunisia: a retrospective study of 46 cases

J Immunoassay Immunochem. 2015;36(2):111-8. doi: 10.1080/15321819.2014.899258.

Abstract

Despite the new available histologic classification of lung cancers, it remains difficult to assess the real prognostic relevance of the different subtypes of lung adenocarcinomas. Facing the prognostic relevance of microvessel density (MVD) and the expression of metalloproteases and thyroid tyrosine factor 1 (TTF1) in different cancers, we tried to compare these parameters in the different subtypes of lung adenocarcinomas. We conducted a retrospective study of 46 adenocarcinomas surgically resected. An immunohistochemical study using the cluster of differentiation 34 (CD34), TTF1, metalloproteases 2 (MMP2), and metalloproteases 9 (MMP9) antibodies was performed on all samples while MVD was evaluated using CD34 antibody. The analyzed cases included 19 acinar, 7 papillary, 1 lepidic, and 19 solid predominant American Thoracic Classification of lung adenocarcinomas (ASK). The higher MVD levels were observed in papillary and acinar subtypes types and a statically significant difference in the MVD was observed in the different subtypes (p< 0,001). This study didn't show significant statistical results concerning MMP and TTF1 expression but it revealed a tendency to an equal expression of these antibodies in the different subtypes. The difference in the MVD between the different subtypes of adenocarcinoma puts emphasis on their prognostic relevance.

Keywords: bronchial washing; lung adenocarcinoma; microvessel density; non small cell carcinoma; prognosis; sensitivity; specificity; specificity sensitivity.

MeSH terms

  • Adenocarcinoma of Lung
  • Adenocarcinoma* / classification
  • Adenocarcinoma* / metabolism
  • Adenocarcinoma* / pathology
  • Adult
  • Aged
  • Antigens, CD34 / metabolism
  • Female
  • Humans
  • Lung Neoplasms* / classification
  • Lung Neoplasms* / metabolism
  • Lung Neoplasms* / pathology
  • Male
  • Matrix Metalloproteinase 2 / metabolism*
  • Matrix Metalloproteinase 9 / metabolism*
  • Microvessels / pathology*
  • Middle Aged
  • Nuclear Proteins / metabolism*
  • Prognosis
  • Thyroid Nuclear Factor 1
  • Transcription Factors / metabolism*
  • Tunisia

Substances

  • Antigens, CD34
  • Nuclear Proteins
  • Thyroid Nuclear Factor 1
  • Transcription Factors
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9