A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

Org Biomol Chem. 2014 May 21;12(19):3071-9. doi: 10.1039/c3ob42505c.

Abstract

An ADP selective fluorescent probe working in aqueous medium was identified and the change in fluorescence as a function of ADP concentration was standardized. Using this probe, all the steps of glycolysis coupled with ATP/ADP inter-conversion and oxidative breakdown of pyruvate in the mitochondria were monitored and the consumption/production of ATP/ADP at each step was quantified. The quantity of ADP present in the mitochondria, taken from different body parts of a pig, was also determined. It is hypothesized that an appropriate modification of the technique may provide a diagnostic tool for monitoring biochemical pathways as well as for quick estimation of ADP in the mitochondria and other cell organelles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / analysis*
  • Animals
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fructosephosphates / metabolism
  • Glucose / metabolism*
  • Glyceric Acids / metabolism
  • Glycolysis
  • Mitochondria / metabolism
  • Oxidation-Reduction
  • Phosphoenolpyruvate / metabolism
  • Phosphorylation
  • Pyruvic Acid / metabolism
  • Solutions
  • Sus scrofa

Substances

  • Fluorescent Dyes
  • Fructosephosphates
  • Glyceric Acids
  • Solutions
  • Adenosine Diphosphate
  • fructose-6-phosphate
  • Phosphoenolpyruvate
  • 3-phosphoglycerate
  • Pyruvic Acid
  • Glucose