Over-expression of miR-98 in FFPE tissues might serve as a valuable source for biomarker discovery in breast cancer patients

Int J Clin Exp Pathol. 2014 Feb 15;7(3):1166-71. eCollection 2014.

Abstract

The miR-98 is thought to be associated with various cancers. This study was to evaluate the potential predictive value of miR-98 expression in formalin-fixed paraffin-embedded tissue of breast cancer patients. The expression levels of miR-98 were examined in 98 breast cancer patients and 40 cancer-free controls using real-time quantitative PCR. The comparison of miR-98 expression levels between patient and control was performed using the Mann-Whitney test. The miR-98 showed higher expression levels in breast cancer patients compared with cancer free controls (p<0.01). The expression levels of miR-98 were highly correlated with miR24/93/378 in breast cancer patients. The miR-98 exhibited great capability of discriminating between cancer patients and controls by the Receiver-operator characteristic (ROC) curve analysis. The miR-98 was found highly correlated with breast cancer by Univariable logistic regression analysis. These results suggest that over-expression of miR-98 in formalin-fixed paraffin-embedded tissues might serve as a valuable source for biomarker discovery in breast cancer patients.

Keywords: Biomarkers; breast cancer; cancer; miR-98; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Area Under Curve
  • Biomarkers / analysis*
  • Breast Neoplasms / metabolism*
  • Female
  • Formaldehyde
  • Humans
  • MicroRNAs / analysis*
  • Middle Aged
  • Paraffin Embedding
  • ROC Curve
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Tissue Fixation
  • Up-Regulation

Substances

  • Biomarkers
  • MIRN98 microRNA, human
  • MicroRNAs
  • Formaldehyde