Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase

J Virol. 2014 Jul;88(13):7659-62. doi: 10.1128/JVI.00967-14. Epub 2014 Apr 9.

Abstract

APOBEC3 proteins are restriction factors that induce G→A hypermutation in retroviruses during replication as a result of cytidine deamination of minus-strand DNA transcripts. However, the mechanism of APOBEC inhibition of murine leukemia viruses (MuLVs) does not appear to be G→A hypermutation and is unclear. In this report, the incorporation of mA3 in virions resulted in a loss in virion reverse transcriptase (RT) activity and RT fidelity that correlated with the loss of virion-specific infectivity.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cytidine Deaminase / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Moloney murine leukemia virus / enzymology*
  • Moloney murine leukemia virus / pathogenicity
  • RNA-Directed DNA Polymerase / metabolism*
  • Retroviridae Infections / enzymology*
  • Retroviridae Infections / virology
  • Transfection
  • Tumor Virus Infections / enzymology*
  • Tumor Virus Infections / virology
  • Virion / pathogenicity*
  • Virus Assembly
  • Virus Replication

Substances

  • RNA-Directed DNA Polymerase
  • Apobec3 protein, mouse
  • Cytidine Deaminase