High-resolution melting assay for genotyping of IFNL4-associated dinucleotide variant rs368234815

E Galmozzi; F Facchetti; R Perbellini; A Aghemo

doi:10.1111/1469-0691.12637

High-resolution melting assay for genotyping of IFNL4-associated dinucleotide variant rs368234815

Clin Microbiol Infect. 2014 Nov;20(11):O936-8. doi: 10.1111/1469-0691.12637. Epub 2014 May 15.

Authors

E Galmozzi¹, F Facchetti, R Perbellini, A Aghemo

Affiliation

¹ Division of Gastroenterology and Hepatology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.

PMID: 24724563
DOI: 10.1111/1469-0691.12637

Abstract

Interferon-λ4-related dinucleotide variant rs368234815 TT/-G is strongly linked with rs12979860 polymorphism, the most important genetic marker connected to treatment-induced hepatitis C virus clearance. Due to additional baseline information that rs368234815 polymorphism could provide for the management of chronic hepatitis C, we developed and validated a high-resolution melting genotyping assay using 193 patients with chronic hepatitis C.

Keywords: IFNL4; IL28B; RT-PCR; hepatitis C virus; high-resolution melting.

MeSH terms

Antiviral Agents / therapeutic use*
Cohort Studies
Genotype
Genotyping Techniques / methods*
Hepatitis C, Chronic / drug therapy*
Hepatitis C, Chronic / immunology*
Humans
Interleukins / genetics*
Treatment Outcome

Substances

Antiviral Agents
IFNL4 protein, human
Interleukins