Cloning of Plasmodium yoelii genes expressing three different sporozoite-specific antigens

Microb Pathog. 1989 Mar;6(3):227-31. doi: 10.1016/0882-4010(89)90072-7.

Abstract

Genomic DNA isolated from Plasmodium yoelii (strain #17XNL) was prepared by partial digestion and cloned in Escherichia coli TB-1 with pUC18 plasmid. Antigen-producing recombinants were detected by a battery of monoclonal antibodies against antigens of the sporozoite stages. Four clones producing stage-specific sporozoite antigens were identified. One produced P. yoelii circumsporozoite protein, and three produced other P. yoelii sporozoite antigens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antigens, Protozoan / biosynthesis
  • Antigens, Protozoan / genetics*
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / analysis
  • DNA / genetics
  • Electrophoresis, Agar Gel
  • Epitopes / genetics
  • Nucleic Acid Hybridization
  • Plasmodium yoelii / genetics*
  • Plasmodium yoelii / immunology
  • Restriction Mapping

Substances

  • Antigens, Protozoan
  • Epitopes
  • DNA