Preliminary molecular characterizations of Sarcoptes scaibiei (Acari: Sarcoptidae) from farm animals in Egypt

Said Amer; Taher Abd El Wahab; Abd El Naby Metwaly; Jianbin Ye; Dawn Roellig; Yaoyu Feng; Lihua Xiao

doi:10.1371/journal.pone.0094705

Preliminary molecular characterizations of Sarcoptes scaibiei (Acari: Sarcoptidae) from farm animals in Egypt

PLoS One. 2014 Apr 11;9(4):e94705. doi: 10.1371/journal.pone.0094705. eCollection 2014.

Authors

Said Amer¹, Taher Abd El Wahab², Abd El Naby Metwaly², Jianbin Ye³, Dawn Roellig⁴, Yaoyu Feng⁵, Lihua Xiao⁴

Affiliations

¹ Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; Department of Zoology, Kafr El Sheikh University, Kafr El Sheikh, Egypt.
² Animal Health Research Institute, Kafr El Sheikh Provincial Laboratory, Kafr El Sheikh, Egypt.
³ Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai, People's Republic of China.
⁴ Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
⁵ State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai, People's Republic of China.

Abstract

Little is known about the genetic diversity of Sarcoptes scabiei mites in farm animals in Egypt. In this study, we characterized S. scabiei in 25 skin scrapes from water buffalo, cattle, sheep, and rabbits at the nuclear marker ITS2 and mitochondrial markers COX1 and 16S rRNA. Sequences of the ITS2 showed no host segregation or geographical isolation, whereas those of the mitochondrial COX1 and 16S rRNA genes indicated the presence of both host-adapted and geographically segregated populations of S. scabiei. Host adaptation may limit inter-species transmission of. S. scabiei, thus restrict gene flow among S. scabiei from different hosts. This is the first report on the molecular characterization of sarcoptic mites in Egypt. Further genetic studies involving larger numbers of specimens, especially those from humans and companion animals, are needed to understand the molecular epidemiology of sarcoptic mange in Egypt.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Animals, Domestic / parasitology*
Cluster Analysis
Computational Biology
DNA, Intergenic
DNA, Mitochondrial
Databases, Nucleic Acid
Egypt
Molecular Sequence Data
RNA, Ribosomal, 16S
Sarcoptidae / classification*
Sarcoptidae / genetics*

Substances

DNA, Intergenic
DNA, Mitochondrial
RNA, Ribosomal, 16S

Associated data

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Grants and funding

This research was supported by the Arab Fund for Economic and Social Development (Zamalat Program), National Natural Science Foundation of China (Project No. 31110103901), and Centers for Disease Control and Prevention. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.