A cis-encoded sRNA controls the expression of fabH2 in Yersinia

FEBS Lett. 2014 May 21;588(10):1961-6. doi: 10.1016/j.febslet.2014.04.005. Epub 2014 Apr 13.

Abstract

YsrH is a novel cis-encoded sRNA located on the opposite strand to fabH2, which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH-mediated regulation of fabH2 expression was investigated in Yersinia pseudotuberculosis. Constitutive and inducible over-expression of YsrH decreased the mRNA level of fabH2, while expression of downstream fabD and fabG remained unaffected. Polynucleotide phosphorylase (PNPase) also played an important role in this regulation process by mediating YsrH decay in the exponential phase. Thus, our data defines a cis-encoded sRNA that regulates fatty acid synthesis via a regulatory mechanism also involving PNPase.

Keywords: 3-oxoacyl-ACP synthase (fabH2); PNPase; Yersinia; Yersinia sRNA regulator of fabH2 (YsrH); cis-Encoded sRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / genetics*
  • Bacterial Proteins / genetics*
  • Blotting, Northern
  • Gene Expression Regulation, Bacterial*
  • Mutation
  • Operon
  • Polyribonucleotide Nucleotidyltransferase / metabolism
  • RNA Stability
  • RNA, Bacterial / genetics*
  • RNA, Bacterial / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Untranslated / genetics*
  • RNA, Small Untranslated / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Yersinia pseudotuberculosis / enzymology
  • Yersinia pseudotuberculosis / genetics*

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • RNA, Messenger
  • RNA, Small Untranslated
  • 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
  • Polyribonucleotide Nucleotidyltransferase