Abstract
Specific immunotherapy (SIT) is the only specific remedy for the treatment of allergic diseases currently. B cells are important immune cells in the immunity. The role of B cells in immune regulatory activities has not been fully understood yet. This study aims to elucidate the role of the thrombospondin (TSP)1-producing B cells in the immune regulatory role of SIT. The results showed that after SIT, the frequency of CD35(+) B cells was increased in the intestine of mice with food allergy. The CD35(+) B cells expressed TSP1 after exposure to specific antigens. Co-culture with the TSP1-producing CD35(+) B cells decreased the levels of CD80/CD86 in dendritic cells; the cells convert naïve CD4(+) T cells to regulatory T cells to inhibit allergic inflammation in the intestine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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B7-1 Antigen / metabolism
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B7-2 Antigen / metabolism
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Cholera Toxin / immunology
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Dendritic Cells / immunology
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Food Hypersensitivity / immunology*
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Immune Tolerance / immunology
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Immunotherapy / methods*
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Inflammation / immunology
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Interleukin-13 / metabolism
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Interleukin-4 / metabolism
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Interleukin-5 / metabolism
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Intestines / cytology
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Intestines / immunology
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Lymphocyte Activation / immunology
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Mice
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Mice, Inbred BALB C
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Ovalbumin / immunology
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Receptors, Complement 3b / biosynthesis
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Receptors, Complement 3b / immunology
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T-Lymphocytes, Regulatory / cytology
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T-Lymphocytes, Regulatory / immunology*
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Thrombospondin 1 / biosynthesis*
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Thrombospondin 1 / immunology
Substances
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B7-1 Antigen
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B7-2 Antigen
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Cd86 protein, mouse
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Interleukin-13
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Interleukin-5
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Receptors, Complement 3b
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Thrombospondin 1
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Thbs1 protein, mouse
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Interleukin-4
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Ovalbumin
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Cholera Toxin