Cellular transcriptome analysis reveals differential expression of pro- and antiapoptosis genes by varicella-zoster virus-infected neurons and fibroblasts

J Virol. 2014 Jul;88(13):7674-7. doi: 10.1128/JVI.00500-14. Epub 2014 Apr 16.

Abstract

Transcriptional changes following varicella-zoster virus (VZV) infection of cultured human neurons derived from embryonic stem cells were compared to those in VZV-infected human foreskin fibroblasts. Transcription of 340 neuronal genes significantly altered by VZV infection included 223 transcript changes unique to neurons. Strikingly, genes inhibiting apoptosis were upregulated in neurons, while proapoptotic gene transcription was increased in fibroblasts. These data are a basis for discovery of differences in virus-host interactions between these VZV targets.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Biomarkers / metabolism*
  • Cells, Cultured
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism*
  • Embryonic Stem Cells / virology
  • Fibroblasts / cytology
  • Fibroblasts / metabolism*
  • Fibroblasts / virology
  • Gene Expression Profiling*
  • Herpesvirus 3, Human / physiology*
  • Humans
  • Neurons / cytology
  • Neurons / metabolism*
  • Neurons / virology
  • Oligonucleotide Array Sequence Analysis

Substances

  • Biomarkers