Flow cytometry in mastocytosis: utility as a diagnostic and prognostic tool

Immunol Allergy Clin North Am. 2014 May;34(2):297-313. doi: 10.1016/j.iac.2014.01.008.

Abstract

This article presents information for the identification and characterization of mast cells from bone marrow and other tissues using multiparametric flow cytometry. In addition, it provides guidelines for the application of this technique in the subclassification of systemic mastocytosis and assessment of the long-term prognosis of patients individually.

Keywords: Bone marrow; Cell purification; Classification; Diagnosis; Flow cytometry; Mast cells; Mastocytosis; Prognosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adult
  • Antigens, CD / genetics
  • Antigens, CD / immunology
  • Bone Marrow / immunology
  • Bone Marrow / pathology*
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Immunophenotyping
  • Mast Cells / immunology
  • Mast Cells / pathology*
  • Mastocytosis / diagnosis*
  • Mastocytosis / genetics
  • Mastocytosis / immunology
  • Mastocytosis / pathology
  • Mutation
  • Prognosis
  • Proto-Oncogene Proteins c-kit / genetics
  • Proto-Oncogene Proteins c-kit / immunology

Substances

  • Antigens, CD
  • Proto-Oncogene Proteins c-kit