Towards personalized medicine mediated by in vitro virus-based interactome approaches

Int J Mol Sci. 2014 Apr 21;15(4):6717-24. doi: 10.3390/ijms15046717.

Abstract

We have developed a simple in vitro virus (IVV) selection system based on cell-free co-translation, using a highly stable and efficient mRNA display method. The IVV system is applicable to the high-throughput and comprehensive analysis of proteins and protein-ligand interactions. Huge amounts of genomic sequence data have been generated over the last decade. The accumulated genetic alterations and the interactome networks identified within cells represent a universal feature of a disease, and knowledge of these aspects can help to determine the optimal therapy for the disease. The concept of the "integrome" has been developed as a means of integrating large amounts of data. We have developed an interactome analysis method aimed at providing individually-targeted health care. We also consider future prospects for this system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell-Free System
  • Genomics
  • Humans
  • Precision Medicine
  • Protein Interaction Maps
  • Proteins / metabolism
  • RNA, Messenger / metabolism
  • Viruses / metabolism*

Substances

  • Proteins
  • RNA, Messenger