Abstract
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aldehydes / chemistry*
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Green Fluorescent Proteins / chemistry*
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Green Fluorescent Proteins / genetics*
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Hydroxylamine / analysis
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RNA, Transfer / analysis
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RNA, Transfer / genetics
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Staining and Labeling / methods*
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Time Factors
Substances
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Aldehydes
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Green Fluorescent Proteins
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Hydroxylamine
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RNA, Transfer