Abstract
We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Boron Compounds* / chemical synthesis
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Boron Compounds* / chemistry
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Cell Nucleus / metabolism*
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Cell Survival
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DNA / chemistry
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Drug Design*
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Fluorescent Dyes* / chemical synthesis
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Fluorescent Dyes* / chemistry
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HeLa Cells
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Humans
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Molecular Imaging / methods*
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Molecular Structure
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Rhodamines* / chemical synthesis
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Rhodamines* / chemistry
Substances
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4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
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Boron Compounds
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Fluorescent Dyes
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Rhodamines
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DNA