Abstract
Cigarette smoking can harm fertility, but the existing research has targeted primarily on ovarian follicles, embryos or sex hormone. In this study, we tested cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression associated with inhibition of oxidative stress using C57BL/6 mice. Mice in the experimental group were administered a cigarette smoke extract (CSE) solution (2 mg/ml) orally daily, while the blank control group was given dimethylsulfoxide (DMSO). A positive control group (menadione) was used that received an intraperitoneal injection of 15 mg/kg menadione in oil solution daily. We found that the CSE group manifested a reduced diameter of zona pellucida-free oocyte (ZP-free OD) and a morphologically misshapen first polar body (PB). Our results suggest that CSE exposure is associated with a shrink size and poor quality of oocytes. Quitting smoking is a wise choice to ensure good fertility.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carcinogens / toxicity*
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Female
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Gene Expression Regulation / drug effects*
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Glutathione / biosynthesis
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Glutathione Transferase / metabolism
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Mice
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Oocytes / cytology
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Oocytes / drug effects*
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Oocytes / metabolism
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Ovary / drug effects*
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Ovary / metabolism
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Ovulation / drug effects*
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Polar Bodies / cytology
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Polar Bodies / drug effects
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Smoke / analysis*
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Tobacco Products / analysis*
Substances
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Carcinogens
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Smoke
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Glutathione Transferase
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Glutathione
Grants and funding
The source of funding that has supported the authors' study is from a scientific research project, "the study of the key technology of increasing the pregnancy rate and security of IVF-ET", of the Technological and Information Bureau of Guangzhou. The grant number is 2012Y2-00022. The URL of the funder's website is
http://www.gzsi.gov.cn/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.