Detachment and flow cytometric quantification of seagrass-associated bacteria

Stacey Trevathan-Tackett; Peter Macreadie; Peter Ralph; Justin Seymour

doi:10.1016/j.mimet.2014.04.008

Detachment and flow cytometric quantification of seagrass-associated bacteria

J Microbiol Methods. 2014 Jul:102:23-5. doi: 10.1016/j.mimet.2014.04.008. Epub 2014 Apr 26.

Authors

Stacey Trevathan-Tackett¹, Peter Macreadie¹, Peter Ralph², Justin Seymour¹

Affiliations

¹ Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia.
² Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia. Electronic address: [email protected].

PMID: 24780150
DOI: 10.1016/j.mimet.2014.04.008

Abstract

A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.

Keywords: Bacterial detachment; Enumeration; Flow cytometry; Microbe; Seagrass; Seagrass-associated bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / isolation & purification*
Bacterial Load / methods*
Detergents / pharmacology
Flow Cytometry / methods*
Polysorbates / pharmacology
Stress, Mechanical
Zosteraceae / microbiology*

Substances

Detergents
Polysorbates