Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel

U B Kaupp; T Niidome; T Tanabe; S Terada; W Bönigk; W Stühmer; N J Cook; K Kangawa; H Matsuo; T Hirose

doi:10.1038/342762a0

Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel

Nature. 1989 Dec 14;342(6251):762-6. doi: 10.1038/342762a0.

Authors

U B Kaupp¹, T Niidome, T Tanabe, S Terada, W Bönigk, W Stühmer, N J Cook, K Kangawa, H Matsuo, T Hirose, et al.

Affiliation

¹ Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.

PMID: 2481236
DOI: 10.1038/342762a0

Abstract

The complete amino-acid sequence of the cyclic GMP-gated channel from bovine retinal rod photoreceptors, deduced by cloning and sequencing its complementary DNA, shows that the protein contains several putative transmembrane segments, followed by a region that is similar to the cyclic GMP-binding domains of cyclic GMP-dependent protein kinase. Expression of the complementary DNA produces cyclic GMP-gated channel activity in Xenopus oocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
Cyclic GMP / physiology*
Cyclic Nucleotide-Gated Cation Channels
DNA / genetics
Eye Proteins / genetics*
In Vitro Techniques
Ion Channel Gating
Ion Channels / physiology*
Membrane Proteins / genetics
Molecular Sequence Data
Protein Conformation
RNA, Messenger / genetics
Rod Cell Outer Segment
Solubility
Xenopus laevis

Substances

Cyclic Nucleotide-Gated Cation Channels
Eye Proteins
Ion Channels
Membrane Proteins
RNA, Messenger
DNA
Cyclic GMP