Objective: To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells.
Methods: The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting.
Results: The infective recombinant baculovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells.
Conclusion: The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.