Abstract
A rationally designed small-molecule fluorogenic probe for nitric oxide (NO) detection based on a new switching mechanism has been developed. Attaching a NO-responsive dihydropyridine pendant group to a fluorophore led to a probe that displays a very high sensitivity to NO concentrations down to the low nM range and a very high specificity to NO while being insensitive to other oxidative oxygen/nitrogen species that often interfere with the sensing of NO.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Line, Tumor
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Coumarins / chemical synthesis
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Coumarins / chemistry*
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Dihydropyridines / chemistry
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Electron Transport
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Hydrogen-Ion Concentration
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Mice
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Microscopy, Fluorescence*
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Niacin / analogs & derivatives*
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Niacin / chemical synthesis
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Niacin / chemistry
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Nitric Oxide / analysis*
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Reactive Nitrogen Species / chemistry
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Reactive Oxygen Species / chemistry
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Spectrometry, Fluorescence*
Substances
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Coumarins
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Dihydropyridines
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Fluorescent Dyes
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Reactive Nitrogen Species
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Reactive Oxygen Species
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Niacin
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Nitric Oxide
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1,4-dihydropyridine