Abstract
We developed an optimized ensemble of aptamers and antibodies that functions as a multivalent adhesive domain for the capture and isolation of cancer cells. When incorporated into a microfluidic device, the ensemble showed not only high capture efficiency, but also superior capture selectivity at a high shear stress (or high flow rate).
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Neoplasm / chemistry*
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Aptamers, Nucleotide / chemical synthesis*
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Cell Separation / methods
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Humans
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Leukemia / blood
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Microfluidic Analytical Techniques
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / immunology
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Neoplasms / pathology*
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Neoplastic Cells, Circulating / pathology*
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Shear Strength
Substances
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Antibodies, Neoplasm
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Aptamers, Nucleotide
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Neoplasm Proteins