Abstract
Membrane-anchored serine proteases serve as important regulators of multiple developmental and homoeostatic processes in mammals. TMPRSS13 (transmembrane protease, serine 13; also known as mosaic serine protease large-form, MSPL) is a membrane-anchored serine protease with unknown biological functions. In the present study, we used mice with the Tmprss13 gene disrupted by a β-galactosidase-neomycin fusion gene insertion to study the expression and function of the membrane-anchored serine protease. High levels of Tmprss13 expression were found in the epithelia of the oral cavity, upper digestive tract and skin. Compatible with this expression pattern, Tmprss13-deficient mice displayed abnormal skin development, leading to a compromised barrier function, as measured by the transepidermal fluid loss rate of newborn mice. The present study provides the first biological function for the transmembrane serine protease TMPRSS13.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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Crosses, Genetic
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Epidermal Cells
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Epidermis / embryology
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Epidermis / metabolism*
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Epidermis / pathology
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Gene Expression Regulation, Developmental*
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Gene Expression Regulation, Enzymologic*
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Heterozygote
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Membrane Proteins / deficiency
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Mice, 129 Strain
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Mice, Inbred C57BL
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Mice, Knockout
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Mouth Mucosa / cytology
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Mouth Mucosa / embryology
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Mouth Mucosa / metabolism
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Mouth Mucosa / pathology
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Mucous Membrane / cytology
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Mucous Membrane / embryology
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Mucous Membrane / metabolism
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Mucous Membrane / pathology
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Recombinant Fusion Proteins / metabolism
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Serine Endopeptidases / deficiency
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Serine Endopeptidases / genetics
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Serine Endopeptidases / metabolism*
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Serine Proteases / drug effects
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Serine Proteases / genetics
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Serine Proteases / metabolism*
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Upper Gastrointestinal Tract / cytology
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Upper Gastrointestinal Tract / embryology
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Upper Gastrointestinal Tract / metabolism
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Upper Gastrointestinal Tract / pathology
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Urinary Bladder / cytology
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Urinary Bladder / embryology
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Urinary Bladder / metabolism
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Urinary Bladder / pathology
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Water-Electrolyte Balance*
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Water-Electrolyte Imbalance / embryology
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Water-Electrolyte Imbalance / genetics
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Water-Electrolyte Imbalance / metabolism
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Water-Electrolyte Imbalance / pathology
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Membrane Proteins
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Recombinant Fusion Proteins
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beta-Galactosidase
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Serine Proteases
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Serine Endopeptidases
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Tmprss13 protein, mouse