A novel application of pulsed electric field (PEF) processing for improving glutathione (GSH) antioxidant activity

Food Chem. 2014 Oct 15:161:361-6. doi: 10.1016/j.foodchem.2014.04.027. Epub 2014 Apr 13.

Abstract

Glutathione (GSH) was treated by pulsed electric field (PEF) processing to investigate its effect on antioxidant activity. The antioxidant activity of GSH was evaluated using 2,2-diphenyl-1-picrylhydrazy (DPPH) radical inhibition. A Box-Behnken design (BBD) with three independent variables, which were concentration, electric field intensity and pulse frequency was used to establish the regression equation of second-order response surface. Optimal conditions were as follows: GSH concentration 8.86mg/mL, electric field intensity 9.74kV/cm and pulse frequency 2549.08Hz. The DPPH radical inhibition increased from 81.83% to 97.40%. Near-infrared spectroscopy (NIR) and mid-infrared spectroscopy (MIR) were used to analyse the change of structure and functional groups of GSH.

Keywords: Analysis of structure and functional groups; Glutathione (GSH); Improvement antioxidant activity; Pulsed electric field (PEF); Response surface methodology (RSM).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Biphenyl Compounds / chemistry
  • Electricity*
  • Food Handling / methods*
  • Glutathione / pharmacology*
  • Picrates / chemistry
  • Spectroscopy, Near-Infrared

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Picrates
  • 1,1-diphenyl-2-picrylhydrazyl
  • Glutathione