Abstract
We have previously shown that SSYA10-001 blocks severe acute respiratory syndrome coronavirus (SARS-CoV) replication by inhibiting SARS-CoV helicase (nsp13). Here, we show that SSYA10-001 also inhibits replication of two other coronaviruses, mouse hepatitis virus (MHV) and Middle Eastern respiratory syndrome coronavirus (MERS-CoV). A putative binding pocket for SSYA10-001 was identified and shown to be similar in SARS-CoV, MERS-CoV, and MHV helicases. These studies show that it is possible to target multiple coronaviruses through broad-spectrum inhibitors.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antiviral Agents / chemistry
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Antiviral Agents / pharmacology*
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Binding Sites
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Cell Line
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Chlorocebus aethiops
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DNA Helicases / antagonists & inhibitors*
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DNA Helicases / chemistry
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Dose-Response Relationship, Drug
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Fibroblasts / drug effects
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Fibroblasts / pathology
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Fibroblasts / virology
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Humans
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Inhibitory Concentration 50
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Mice
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Middle East Respiratory Syndrome Coronavirus / drug effects*
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Middle East Respiratory Syndrome Coronavirus / physiology
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Molecular Docking Simulation
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Molecular Sequence Data
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Murine hepatitis virus / drug effects*
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Murine hepatitis virus / physiology
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Protein Binding
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Sequence Alignment
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Sequence Homology, Amino Acid
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Severe acute respiratory syndrome-related coronavirus / drug effects*
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Severe acute respiratory syndrome-related coronavirus / physiology
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Triazoles / chemistry
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Triazoles / pharmacology*
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Vero Cells
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Viral Proteins / antagonists & inhibitors*
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Viral Proteins / chemistry
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Virus Replication / drug effects
Substances
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Antiviral Agents
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SSYA10-001
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Triazoles
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Viral Proteins
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DNA Helicases