Detection of opsonic antibodies against Enterococcus faecalis cell wall carbohydrates in immune globulin preparations

Infection. 2014 Aug;42(4):749-55. doi: 10.1007/s15010-014-0628-y. Epub 2014 May 23.

Abstract

Three different commercially available polyvalent immune globulins (IG) were investigated for the existence of antibodies against cell wall carbohydrates of four different E. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). All three IG preparations contained antibodies against all four serotypes (CPS-A to CPS-D). However, only one of the three IG preparations showed opsonic killing against all four serotypes. Average killing was higher against serotypes A and B (72 and 79 %, respectively) than against serotypes C and D (30 and 37 %, respectively). Such IG preparations could play a role as an adjuvant therapeutic option in life-threatening infections with E. faecalis, particularly when resistant strains are involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / immunology*
  • Cell Wall / immunology*
  • Enterococcus faecalis / classification
  • Enterococcus faecalis / immunology*
  • Enterococcus faecalis / physiology
  • Gram-Positive Bacterial Infections / therapy
  • Humans
  • Immunoglobulins / immunology*
  • Immunotherapy / methods
  • Microbial Viability
  • Opsonin Proteins / immunology*
  • Pharmaceutical Preparations*
  • Serogroup

Substances

  • Carbohydrates
  • Immunoglobulins
  • Opsonin Proteins
  • Pharmaceutical Preparations