Assessing regulated nuclear transport in Saccharomyces cerevisiae

Methods Cell Biol. 2014:122:311-30. doi: 10.1016/B978-0-12-417160-2.00014-X.

Abstract

Regulated protein transport between the cytoplasm and the nucleoplasm occurs through nuclear pore complexes and is critical to the function of numerous biological pathways. Saccharomyces cerevisiae has been used as a model system to probe the underlying mechanisms of nuclear transport and how they regulate various physiological processes. This has been facilitated, in part, by studies that couple the microscopic observation of a fluorescently tagged transport cargo's in vivo localization with numerous genetic and biochemical tools available to yeast researchers. Here, we describe some of these methods as they pertain to studies on regulated nuclear transport.

Keywords: Cell cycle; Karyopherins; Nuclear pore complex; Saccharomyces cerevisiae; nuclear transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / genetics*
  • Amino Acid Motifs
  • Cell Cycle Checkpoints / genetics
  • Exportin 1 Protein
  • Gene Expression / genetics
  • Green Fluorescent Proteins
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism
  • Karyopherins / genetics
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism
  • Microscopy, Fluorescence / methods
  • Monomeric GTP-Binding Proteins / genetics
  • Monomeric GTP-Binding Proteins / metabolism
  • Mutation
  • Nuclear Pore / metabolism*
  • Nuclear Pore Complex Proteins / metabolism
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic / genetics
  • Protein Structure, Tertiary
  • Protein Transport / genetics*
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction
  • Temperature
  • alpha Karyopherins / metabolism
  • beta Karyopherins / metabolism

Substances

  • GSP1 protein, S cerevisiae
  • Guanine Nucleotide Exchange Factors
  • Karyopherins
  • Membrane Transport Proteins
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • PSE1 protein, S cerevisiae
  • Receptors, Cytoplasmic and Nuclear
  • SRM1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • alpha Karyopherins
  • beta Karyopherins
  • Green Fluorescent Proteins
  • Monomeric GTP-Binding Proteins