Investigation of maternal and fetal exposure to an IgG2 monoclonal antibody following biweekly intravaginal administration to cynomolgus monkeys throughout pregnancy

Reprod Toxicol. 2014 Sep:48:132-7. doi: 10.1016/j.reprotox.2014.05.003. Epub 2014 May 22.

Abstract

To assess the potential for male-mediated drug transfer to their female partner and/or developing conceptus, vaginal uptake of a monoclonal antibody (mAb) biotherapeutic was assessed in cynomolgus monkeys. A human IgG2 mAb (IgG2X; bound human and cynomolgus monkey neonatal Fc-receptor, FcRn, with similar high affinity) was administered intravaginally (IvG; 100mg/dose) to 5 pregnant cynomolgus monkeys biweekly from gestation day (gd) 21 to gd133. In all maternal samples collected before gd119, IgG2X plasma concentrations were below the limit of quantification (BLQ; <25ng/mL). After dosing on gd119 and 133, maternal IgG2X plasma concentrations remained BLQ in 3/5 monkeys and were very low in 2/5 (up to 116ng/mL; ∼0.01% of the IvG dose). IgG2X was BLQ in all fetal plasma samples. These data indicate that male-mediated mAb drug transfer via seminal fluid does not present a health risk to the female partner and is not bioavailable to the developing conceptus.

Keywords: Human risk; Monoclonal antibody; Vaginal absorption.

MeSH terms

  • Administration, Intravaginal
  • Animals
  • Antibodies, Monoclonal / administration & dosage*
  • Antibodies, Monoclonal / blood
  • Antibodies, Monoclonal / pharmacokinetics*
  • Female
  • Fetal Blood / metabolism*
  • Immunoglobulin G / administration & dosage*
  • Immunoglobulin G / blood
  • Immunoglobulin G / pharmacology*
  • Macaca fascicularis
  • Male
  • Maternal Exposure
  • Maternal-Fetal Exchange
  • Pregnancy
  • Semen / metabolism

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin G