Fabrication of biconcave discoidal silica capsules and their uptake behavior by smooth muscle cells

Shupeng She; Dahai Yu; Xu Han; Weijun Tong; Zhengwei Mao; Changyou Gao

doi:10.1016/j.jcis.2014.03.062

Fabrication of biconcave discoidal silica capsules and their uptake behavior by smooth muscle cells

J Colloid Interface Sci. 2014 Jul 15:426:124-30. doi: 10.1016/j.jcis.2014.03.062. Epub 2014 Apr 2.

Authors

Shupeng She¹, Dahai Yu¹, Xu Han¹, Weijun Tong², Zhengwei Mao¹, Changyou Gao³

Affiliations

¹ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
² MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: [email protected].
³ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: [email protected].

PMID: 24863774
DOI: 10.1016/j.jcis.2014.03.062

Abstract

Biconcave discoidal silica microcapsules were fabricated by reaction of tetraethoxysilane (TEOS) on biconcave discoidal Ca(OH)2 templates, followed by core removal. The biconcave discoidal morphology of microcapsules was characterized by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). The thickness of silica capsule shell can be tuned by two methods, "Gradient concentration" method and "Multi-step growth" method. Through the latter one, the shell thickness can be controlled more effectively. Compared with spherical microcapsules, the biconcave discoidal ones were internalized into smooth muscle cells (SMCs) with a slower rate.

Keywords: Biconcave discoidal; Cellular uptake; Silica microcapsules; Sol–gel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Muscle, Smooth / cytology
Muscle, Smooth / metabolism*
Silicon Dioxide / chemistry*
X-Ray Diffraction

Substances

Silicon Dioxide